Hordein gene expression in a low protein barley cultivar.

Effect of nitrogen nutrition on endosperm protein synthesis in wild and cultivated barley grown in spike culture.

In normal growth conditions, total protein percent (salt soluble plus hordein fractions) in the endosperm at maturity in barley cultivar Hordeum vulgare L. cv ;Ruth' was about 14%, whereas in an accession of wild barley, Hordeum spontaneum Koch line 297, it was about 28%. Spike culture experiments were conducted to ascertain whether there were basic differences between the two genotypes under c...

Nucleotide sequence of a B1 hordein gene and the identification of possible upstream regulatory elements in endosperm storage protein genes from barley, wheat and maize.

The B-hordeins are the major group of prolamin storage proteins in barley (Hordeum vulgare L.) and they are encoded by a small multigene family that is expressed specifically in the developing endosperm. We report the complete nucleotide sequence of a clone of one B-hordein gene (pBHR184). The cloned gene contains no introns and belongs to the B1 sub-family of B-hordein genes. Comparison of the...

Identification and evaluation of HvPIP1; 4 and HvnsLTP genes expression for drought tolerance in barley

It is of great significance to understand the tolerance mechanisms by which plants deal with drought stress and application of these mechanisms for improvement of genotypes in response to drought stress. In order to identify and investigate the expression of genes involved in tolerance to drought stress, leaf and root EST were analyzed in Spontaneum (wild barley) and Nimruz (tolernt to drought ...

Cloning and Expression of the Coat Protein Gene of Barley Yellow Dwarf Virus-PAV in Escherichia coli

Influence of Nitrogen Nutrition on the Amount of Hordein, Protein Z and I3-amylase Messenger Rna in Developing Endosperms of Barley

The amount ofmRNA encoding hordein, protein Z and !3-amylase was analyzed in developing endosperms of the barley variety Bomi and the high-lysine mutant Riso 56. Spikes were cultured in liquid media containing different amounts of ammonium nitrate and endosperms harvested between 15 and 25 days after anthesis. Levels of mRNA were determined by in vitro translations. In addition, B hordein and p...